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Effect of intestinal ischemia/reperfusion injury on leptin and orexin-A levels
LIN Ji, YAN Guangtao, HAO Xiuhua, ZHANG Kai, GAO Xiaoning, LIAO Jie
《医学前沿(英文)》 2007年 第1卷 第1期 页码 87-92 doi: 10.1007/s11684-007-0017-3
关键词: significant difference intestinal I/R transcriptase-polymerase metabolic central secretory
Altered intestinal microbiota associated with colorectal cancer
Hong Zhang, Ying Chang, Qingqing Zheng, Rong Zhang, Cheng Hu, Weiping Jia
《医学前沿(英文)》 2019年 第13卷 第4期 页码 461-470 doi: 10.1007/s11684-019-0695-7
关键词: colorectal cancer (CRC) gut microbiota intestinal Eubacterium Devosia
Inhibition of NO emission by adding antioxidant mixture in
PRABU,R. B. ANAND
《能源前沿(英文)》 2015年 第9卷 第2期 页码 238-245 doi: 10.1007/s11708-015-0356-8
关键词: NO emission antioxidants Succinimide N N-dimethyl-p-phenylenediamine-dihydrochloride N-phenyl-p-phenylenediamine
A photolysis coefficient for characterizing the response of aqueous constituents to photolysis
David R. HOKANSON,Ke LI,R. Rhodes TRUSSELL
《环境科学与工程前沿(英文)》 2016年 第10卷 第3期 页码 428-437 doi: 10.1007/s11783-015-0780-3
关键词: UV photolysis disinfection advanced oxidation N-nitrosodimethylamine quantum yield absorption coefficient
ADT-OH improves intestinal barrier function and remodels the gut microbiota in DSS-induced colitis
《医学前沿(英文)》 页码 972-992 doi: 10.1007/s11684-023-0990-1
关键词: inflammatory bowel disease ADT-OH intestinal permeability gut microbiota
Kinematical synthesis of an inversion of the double linked fourbar for morphing wing applications
AGUIRREBEITIA, R. AVILéS, I. FERNáNDEZ, M. ABASOLO
《机械工程前沿(英文)》 2013年 第8卷 第1期 页码 17-32 doi: 10.1007/s11465-013-0364-5
This paper presents the kinematical features of an inversion of the double linked fourbar for morphing wing purposes. The structure of the mechanism is obtained using structural synthesis concepts, from an initial conceptual schematic. Then, kinematic characteristics as instant center of rotation, lock positions, dead point positions and uncertainty positions are derived for this mechanism in order to face the last step, the dimensional synthesis; in this sense, two kinds of dimensional synthesis are arranged to guide the wing along two positions, and to fulfill with the second one some aerodynamic and minimum actuation energy related issues.
关键词: morphing wing structural synthesis dimensional synthesis geometrical kinematics
Vahideh R. Hokmabad, Soodabeh Davaran, Marziyeh Aghazadeh, Effat Alizadeh, Roya Salehi, Ali Ramazani
《化学科学与工程前沿(英文)》 2019年 第13卷 第1期 页码 108-119 doi: 10.1007/s11705-018-1742-7
Plants have been used for medicinal purposes for thousands of years but they are still finding new uses in modern times. For example, Elaeagnus angustifolia (EA) is a medicinal herb with antinociceptive, anti-inflammatory, antibacterial and antioxidant properties and it is widely used in the treatment of rheumatoid arthritis and osteoarthritis. EA extract was loaded onto poly(ϵ-caprolactone)-poly(ethylene glycol)-poly(ϵ-caprolactone) (PCL-PEG-PCL/EA) nanofibers and their potential applications for bone tissue engineering were studied. The morphology and chemical properties of the fibers were evaluated using Fourier transform infrared spectroscopy, field emission scanning electron microscopy, contact angle measurements and mechanical tests. All the samples had bead-free morphologies with average diameters ranging from 100 to 200 nm. The response of human cells to the PCL-PEG-PCL/EA nanofibers was evaluated using human dental pulp stem cells (hDPSCs). The hDPSCs had better adhesion and proliferation capacity on the EA loaded nanofibers than on the pristine PCL-PEG-PCL nanofibers. An alizarin red S assay and the alkaline phosphatase activity confirmed that the nanofibrous scaffolds induced osteoblastic performance in the hDPSCs. The quantitative real time polymerase chain reaction results confirmed that the EA loaded nanofibrous scaffolds had significantly upregulated gene expression correlating to osteogenic differentiation. These results suggest that PCL-PEG-PCL/EA nanofibers might have potential applications for bone tissue engineering.
关键词: Elaeagnus angustifolia scaffold electrospinning human dental pulp stem cell tissue engineering
R158Q and G212S, novel pathogenic compound heterozygous variants in of Gitelman syndrome
《医学前沿(英文)》 2022年 第16卷 第6期 页码 932-945 doi: 10.1007/s11684-022-0963-9
关键词: Gitelman syndrome mouse model compound heterozygous hypokalemia Slc12a3
基于系统发育和比较基因组分析揭示与发酵乳杆菌缓解结肠炎相关的关键基因 Article
赵岩, 张程程, 于雷雷, 田丰伟, 赵建新, 张灏, 陈卫, 翟齐啸
《工程(英文)》 2022年 第17卷 第10期 页码 170-182 doi: 10.1016/j.eng.2020.09.016
越来越多的研究表明,发酵乳杆菌可以用于溃疡性结肠炎的预防和治疗。本研究中,我们从中国不同地区的人群粪便样本中分离出了105 株发酵乳杆菌,并对其基因组草图进行了测序。我们分析了这些菌株的泛基因组和系统发育特征,并对4 个模型菌株(发酵乳杆菌3872、CECT5716、IFO3956 和VRI003)也进行了分析。系统发育分析表明,发酵乳杆菌基因组的进化方向与宿主的地理位置、性别、族群和年龄没有明显的关系。我们挑选了3 株来自不同的系统发育支系的发酵乳杆菌(FWXBH115、FGDLZR121和FXJCJ61)和发酵乳杆菌模式菌株CECT5716,通过构建右旋糖酐硫酸钠(DSS)诱导的结肠炎小鼠模型,
探究这几株菌的抗炎和免疫调节活性。发酵乳杆菌FXJCJ61 和CECT5716 可以通过缓解所有结肠炎相关的组织学指标,保护黏膜完整性,增加肠道短链脂肪酸(SCFA),显著减轻结肠炎,而其他两株菌未能提供类似的保护作用。发酵乳杆菌FXJCJ61 和CECT5716 的抗炎机制与核转录因子kappa-B(NF-κB)信号通路激活以及促进白细胞介素10(IL-10)的产生有关。比较基因组分析结果表明,这些有益发酵乳杆菌的抗炎作用可能与一些特定基因有关。
SANTOS,Jorge I. FAJARDO,Alvaro R. CUJI,Jaime A. GARCÍA,Luis E. GARZÓN,Luis M. LÓPEZ
《机械工程前沿(英文)》 2015年 第10卷 第3期 页码 287-293 doi: 10.1007/s11465-015-0346-x
A polymeric natural fiber-reinforced composite is developed by extrusion and injection molding process. The shrinkage and warpage of high-density polyethylene reinforced with short natural fibers of Guadua angustifolia Kunth are analyzed by experimental measurements and computer simulations. Autodesk Moldflow® and Solid Works® are employed to simulate both volumetric shrinkage and warpage of injected parts at different configurations: 0 wt.%, 20 wt.%, 30 wt.% and 40 wt.% reinforcing on shrinkage and warpage behavior of polymer composite. Become evident the restrictive effect of reinforcing on the volumetric shrinkage and warpage of injected parts. The results indicate that volumetric shrinkage of natural composite is reduced up to 58% with fiber increasing, whereas the warpage shows a reduction form 79% to 86% with major fiber content. These results suggest that it is a highly beneficial use of natural fibers to improve the assembly properties of polymeric natural fiber-reinforced composites.
关键词: biocomposite natural fiber shrinkage simulation warpage
基于同一健康策略探究老挝人、动物和环境中多黏菌素耐药性的传播特征 Article
Dance, Brekhna Hassan, Timothy R. Walsh
《工程(英文)》 2022年 第15卷 第8期 页码 45-56 doi: 10.1016/j.eng.2022.01.013
This study was designed to investigate the molecular epidemiology of mobile colistin resistance (mcr) using a "One-Health" approach in Laos and to predict whether any dominant plasmid backbone and/or strain type influences the dissemination of mcr. We collected 673 samples from humans (rectal normal flora), poultry, and the environment (water, flies, birds, etc.) in Vientiane, Lao People's Democratic Republic (Laos), from May to September 2018. A total of 238 Escherichia coli (E. coli) isolated from nonduplicative samples, consisting of 98 MCR-positive E. coli (MCRPEC) ("mcr" denotes the gene encoding mobile colistin resistance, and "MCR" denotes the subsequent protein encoded by mcr) and 140 MCRnegative E. coli (MCRNEC), were characterized by phenotype and Illumina sequencing. A subset of MCRPEC was selected for MinION sequencing, conjugation assay, plasmid stability, and growth kinetics in vitro. The prevalence of MCRPEC was found to be 14.6% (98/673), with the highest prevalence in human rectal swabs (45.9% (45/98), p < 0.0001, odds ratio (OR): 0.125, 95% confidence interval (CI): 0.077–0.202). The percentages of MCRPEC from other samples were 14.3% (2/14) in dog feces, 12.0% (24/200) in flies, 11.0% (11/100) in chicken meat, 8.9% (8/90) in chicken cloacal, 8.0% (4/50) in chicken caeca, and 7.5% (4/53) in wastewater. MCRPEC was significantly more resistant to co-amoxiclav, sulfamethoxazoletrimethoprim, levofloxacin, ciprofloxacin, and gentamicin than MCRNEC (p < 0.05). Genomic analysis revealed the distribution of MCRPEC among diverse clonal types. The putative plasmid Inc types associated with mcr-1 were IncX4, IncHI2, IncP1, IncI2, and IncFIA, and those associated with mcr-3 were IncFII, IncFIA, IncFIB, IncP1, and IncR. Recovery of highly similar plasmids from both flies and other sampling sectors implied the role of flies in the dissemination of mcr-1. mcr-positive plasmids were shown to be conjugative, and a significantly high transfer rate into a hypervirulent clone ST1193 was observed. Plasmids containing mcr irrespective of Inc type were highly stable and invariably did not exert a fitness effect upon introduction into a new host. These findings signify the urgent need for a standard infection control program to radically decontaminate the source of resistance.
碳青霉烯类耐药性在人源和动物源产NDM酶大肠埃希菌间的传播 Article
尹丹丹, 杨璐, 陈义强, 许春燕, 李基云, 蒋君瑶, 王雪杨, 付玉林, 邵东延, 刘德俊, 马藤菲, 蔡畅, 沈张奇, 王少林, 李娟, 张嵘, 柯跃斌, 吴聪明, 沈建忠, Timothy R.
《工程(英文)》 2022年 第15卷 第8期 页码 24-33 doi: 10.1016/j.eng.2021.07.030
Although carbapenem use is prohibited in animals in China, carbapenem-resistant Escherichia coli (CREC), especially New Delhi metallo-β-lactamase (NDM)-producing strains, are widely prevalent in foodproducing animals. At present, the impact of livestock-associated CREC strains on human populations at the national level is unknown. Here, we conduct a retrospective cross-sectional study to investigate the prevalence of CREC from clinical settings across 22 Chinese provinces or municipalities and analyze anthropogenic factors associated with their presence. We also ascertain the blaNDM and blaKPC abundance among pig and chicken farms and present a detailed genomic framework for CREC of animal and human origin. Overall, 631/29799 (2.1%) clinical Escherichia coli (E. coli) isolates were identified as CREC. Multivariable analysis revealed that being male, an age below 1, an age between 13 and 18, provinces with greater chicken production, and provinces with higher pig production were associated with higher odds of CREC infection. In general, 73.8% (n = 45/61) of pig farms and 62.2% (n = 28/45) of chicken farms had a blaNDM abundance of 1×10-5 to 1×10-3 and 1×10-3 to 1×10-2, respectively. Among all the Chinese NDM-positive E. coli (n = 463) available at the National Center for Biotechnology Information (NCBI), the genomic analysis revealed that blaNDM-5 and IncX3 were the predominant carbapenemase gene-plasmid combination, while a highly homogeneous relationship between NDM-positive isolates from humans and animals was demonstrated at the plasmid and core genome levels. All the findings suggest frequent CREC transmission between humans and animals, indicating that further discussions on the use of antibiotics in animals and humans are needed, both in China and across the globe.
关键词: Carbapenem resistance Escherichia coli blaNDM-5 One Health Antimicrobial resistance transmission
Etiology of invasive candidosis agents in Russia: a multicenter epidemiological survey
null
《医学前沿(英文)》 2018年 第12卷 第1期 页码 84-91 doi: 10.1007/s11684-017-0612-x
A multicenter prospective epidemiological survey on the etiologic agents of invasive candidosis was conducted in Russia in the period of 2012–2014. Samples were collected from 284 patients with invasive candidosis and Candida species isolated by culture. The species were identified by DNA sequencing and MALDI-TOF mass-spectrometry. A total of 322 isolates were recovered, in which 96% of Сandida species belonged to six major species, namely, C. albicans (43.2%), C. parapsilosis (20.2%), C. glabrata (11.5%), C. tropicalis (9.6%), C. krusei (6.2%), and C. guilliermondii (5.3%). Most Candida species were isolated from blood samples (83.23%). Notably, the prevalence rate of C. albicans reduced from 52.38% to 32.79% (2012 vs. 2014) (P=0.01) whereas that of non-C. albicans increased from 47.62% (2012) to 67.21% (2014) (P<0.01). Species distribution differed among geographical regions; specifically, the prevalence rate of C. albicans as an etiologic agent of invasive candidosis in Siberian Federal region was significantly higher than that in other Federal regions. Results indicated a shift from C. albicans to non-C. albicans. Therefore, a detailed investigation on the contributing factors and appropriate treatment of invasive candidosis is needed.
关键词: prevalence &Scy andida species invasive candidosis nosocomial etiology MALDI-TOF mass-spectrometry candidemia
通过原位观察揭示人体肠道微生物组的重建和动态变化 Article
刘小林, 戴敏, Yue Ma, 赵娜, Ziyu Wang, Ying Yu, Yakun Xu, Huijie Zhang, Liyuan Xiang, He Tian, 税光厚, 张发明, 王军
《工程(英文)》 2022年 第15卷 第8期 页码 89-101 doi: 10.1016/j.eng.2021.03.015
人体肠道微生物组主要通过使用粪便样本进行研究,这种做法已经得到了关于胃肠道微生物群落的组成和功能的重要知识。然而,这种对粪便材料的依赖限制了对胃肠道其他位置(原位)微生物动力学的研究,并且粪便样本不能随时获得,这也阻碍了在更精细的时间尺度(如小时)下进行分析。在我们的研究中,我们利用结肠途径经内镜肠内导管(一种最初为粪便微生物群移植开发的技术)每天两次对回盲部微生物组进行采样;然后对这些样品进行宏基因组和宏转录组学分析。从5 名健康志愿者身上共收集了43 份回盲部样本及28 份尿液和粪便样本。在5 名志愿者中分析的回盲部和粪便微生物组被发现在宏基因组分析中相似,但它们的活性基因(宏转录组)被发现高度不同。两种微生物组在泻药暴露后都受到干扰;随着时间的推移,它们表现出与治疗前状态的差异减少,从而证明了作为肠道微生物组的先天特性——恢复力,尽管它们在我们的观察时间窗口内没有完全恢复。白天和夜间对回盲部微生物组的采样显示,在一系列细菌种类和功能途径中存在昼夜节律,特别是与短链脂肪酸产生相关的细菌,如痤疮丙酸杆菌和辅酶A生物合成II。自相关分析和波动分解进一步表明了昼夜振荡的显著周期性。粪便和尿液样本中的代谢组学分析反映出了肠道微生物组的扰动和恢复,表明肠道微生物组对参与宿主健康的诸多关键代谢物的重要贡献。这项研究为人体肠道微生物组及其内在恢复力和昼夜节律以及这些对宿主的潜在后果提供了新的见解。
Non-thermal plasma for exhaust gases treatment
Elvia ALVA R.,Marquidia PACHECO P.,Fernando GÓMEZ B.,Joel PACHECO P.,Arturo COLÍN C.
《机械工程前沿(英文)》 2015年 第10卷 第3期 页码 301-305 doi: 10.1007/s11465-015-0344-z
This article describes a study on a non-thermal plasma device to treat exhaust gases in an internal combustion engine. Several tests using a plasma device to treat exhaust gases are conducted on a Honda GX200-196 cm3 engine at different rotational speeds. A plasma reactor could be efficient in degrading nitrogen oxides and particulate matter. Monoxide and carbon dioxide treatment is minimal. However, achieving 1%–3% degradation may be interesting to reduce the emission of greenhouse gases.
标题 作者 时间 类型 操作
Effect of intestinal ischemia/reperfusion injury on leptin and orexin-A levels
LIN Ji, YAN Guangtao, HAO Xiuhua, ZHANG Kai, GAO Xiaoning, LIAO Jie
期刊论文
Altered intestinal microbiota associated with colorectal cancer
Hong Zhang, Ying Chang, Qingqing Zheng, Rong Zhang, Cheng Hu, Weiping Jia
期刊论文
A photolysis coefficient for characterizing the response of aqueous constituents to photolysis
David R. HOKANSON,Ke LI,R. Rhodes TRUSSELL
期刊论文
ADT-OH improves intestinal barrier function and remodels the gut microbiota in DSS-induced colitis
期刊论文
Kinematical synthesis of an inversion of the double linked fourbar for morphing wing applications
AGUIRREBEITIA, R. AVILéS, I. FERNáNDEZ, M. ABASOLO
期刊论文
Effect of incorporating Elaeagnus angustifolia extract in PCL-PEG-PCL nanofibers for bone tissue engineering
Vahideh R. Hokmabad, Soodabeh Davaran, Marziyeh Aghazadeh, Effat Alizadeh, Roya Salehi, Ali Ramazani
期刊论文
Experimental evaluation and simulation of volumetric shrinkage and warpage on polymeric composite short natural fibers reinforced injection molded
SANTOS,Jorge I. FAJARDO,Alvaro R. CUJI,Jaime A. GARCÍA,Luis E. GARZÓN,Luis M. LÓPEZ
期刊论文
碳青霉烯类耐药性在人源和动物源产NDM酶大肠埃希菌间的传播
尹丹丹, 杨璐, 陈义强, 许春燕, 李基云, 蒋君瑶, 王雪杨, 付玉林, 邵东延, 刘德俊, 马藤菲, 蔡畅, 沈张奇, 王少林, 李娟, 张嵘, 柯跃斌, 吴聪明, 沈建忠, Timothy R.
期刊论文
通过原位观察揭示人体肠道微生物组的重建和动态变化
刘小林, 戴敏, Yue Ma, 赵娜, Ziyu Wang, Ying Yu, Yakun Xu, Huijie Zhang, Liyuan Xiang, He Tian, 税光厚, 张发明, 王军
期刊论文